Thinking of buying antibodies against your protein? You should be aware that :
« Not all antibodies sold by companies are practical for all methods. An increasingly frustrating problem in recent years has been the rapid production and sale of poorly characterized antibodies by some commercial houses.1»
When a company claims that an antibody is effective in histochemistry, it does not necessarily mean that this antibody will be as effective in Western Blot. The reality of the ongoing large-scale commercialization is that antibodies cannot be as unique in character as they were once. Essentially, antibody design has lost a pertinent aspect of its artisanal origins.
Biosiris_RA will work to provide you with antibodies uniquely designed for your specific applications.
Never, if you have time, money and expertise to spend at the calibration of your antibody and if can accept to switch your assay to fit the best antibody performance.
Now, if time is short, if you are wasting time on an antibody lacking specificity and if you are stuck to one particular screening assay (Western Blot or immunohisto, or else), in those conditions, we can help you. If your idea is to inject peptidic fragments of your protein to produce antibodies, we can help you.
We shall not produce the antibody but we shall advice you which peptidic fragments should be your best choices.
1 Harlow, E. and Lane, D. (1999). Using antibodies: a laboratory manual. Cold Spring Harbor Laboatory Press, Cold Spring Harbor, New York.
Biosiris_RA organizes training courses on the analysis of protein sequences and structures. These courses include the prediction of B epitopes.
